Hybridization methods are in such wide use in cell biology today that it is difficult. On an electrophoresis gel, band b is closer to the positive end of the gel than is band a. When the plasmid dna digested with combinations of restriction enzymes and the resulting fragments are electrophoresed, the following data is obtained. If you observe the expected sizes, then your design is correct and your analysis are confirmed. The areas comprising the current biotechnology are bioprocess, monoclonal antibodies, and. What is normally the source plasmid for human cloning. If you dont see any interesting for you, use our search form on bottom v. This is great for homework, supplementary busy worksheet for a substitute teacher, or can be used as part. Plasmid definition, types and functions biology dictionary. Using the information from table 4,indicate where the restriction enzyme cuts are located on the plasmid. You will be glad to know that right now biology lab cloning paper plasmid answer pdf is available on our online library. Gene cloning worksheet 20pts recombinant dna is a plasmid that has had foreign genes added to it.
Worksheets with answers plasmid molecular cloning scribd. Because of this effect, these substances are known as. To defend when attacked by a virus, bacteria use chemical weapons that break up the dna of the virus. Save the key about antibiotic resistance, from the bottom of the pink sheet for later use. In this activity students will use a plasmid map to calculate the products of various restriction enzyme digests and predict the location of the dna fragments when run on a gel. Each of the individual worksheets includes target language from the relevant lesson plan in this book. Your recombinant plasmid should be circular with a portion of the cell dna included. In this laboratory investigation, plasmids containing fragments of foreign dna will be used to transform escherichia coli cells, conferring antibiotic ampicillin resistance, as. If you spliced the dna gene into the middle of the plasmid replication site, the plasmid will not be able to replicate, and cannot be of use. Our main purpose is that these 1 rna worksheet answer key photos gallery can be a guide for you, give you more samples and also bring you what you looking for. To help teachers produce multiple colour copies for group classes, all activity worksheets are also available as a full colour pdf download from.
Using this map as a guide, give the number of restriction fragments along with their associated lengths that. Experimental studies in molecular biology with transformed. If the dna is in the form of a circular dna called a plasmid, it can be copied in. Copy number is regulated by initiation of plasmid replication 10. Inserting a plasmid into dna would allow cells to express a protein that they are lacking. Do not let the agar cool so much that is begins to solidify. Students use a paper model to simulate this reallife process used by biotechnicians. Living and nonliving things, fruits and vegetables, parts of a plant diagram, plant life cycle, life cycle of a fish, adaptation of animals, plant adaptation and life cycle of a chicken. Big genetics and information transfer 3 ap central.
Ap biology molecular biology lab bacterial transformation worksheet 417 below is the link to the new freeresponse booklet which shows how questions and space for answers will be distributed throughout the exam booklet. By selecting the box below, i am agreeing to allow addgene, via its ebook service provider, hubspot, to store my email address indefinitely or until i opt out of receiving updates to the plasmids 101 ebook so that i can receive the plasmids 101 ebook and any updates to this ebook at the email address i. Bacteria transformation activitymodeling bacteria transformation worksheet modeling bacteria transformation worksheet data and observations staple recombinant model here. In a molecular biology laboratory, a student obtained competent e. Gel electrophoresis worksheet teachers pay teachers. In this biology learning exercise, students complete 7 short answer questions regarding dna, gene splicing, cloning, and bacterial proteins. Introduction to addgenes resource any newcomer who joins a molecular biology lab will undoubtedly be asked to design, modify, or construct a plasmid. Ms foglia ap biology lab cloning paper plasmid answers. The essence of cell chemistry is to isolate a particular cellular component and then analyze its chemical structure and activity. A double digest means that you cut your plasmid with two different restriction enzymes at the same time. This worksheet is visual and will either help drive a concept home or open their eyes to a new concept. Bacterial transformation with green fluorescent protein pglo.
The percent composition of a compound was found to be 63. Consult the manufacturers manual for the optimal working conditions for each enzyme. After finishing the modification, have teams complete the modeling bacteria transformation worksheet to demonstrate their understanding of the modification process. Identification of bacterial plasmids based on mobility and plasmid. The tools recombinant dna is a set of tools that allows scientists to move between genetics, biochemistry and. Notice how all the fragments add up to the same total. Recombinant dna can be used to create massive amounts of proteins for research, and has also been used to add genes to organisms or cells. The plasmids conveniently can be cut, fused with other dna and then. I will include photos of the completed sequences when i get a chance, for now, just including answers to the analysis questions. After finishing the modification, have teams complete the modeling bacteria transformation worksheet to demonstrate their understanding of the. Describe a procedure to determine which bacterial cells have been successfully transformed. To move the pglo plasmid dna through the cell membrane you will. Read a short article about how restriction enzymes are used to cut bits of dna and those bits can be inserted into the genome of other organisms. Biomedical application of plasmid dna in gene therapy.
A virus or plasmid used in this way is known as a cloning vector, and the dna. As you already have a putative map of the plasmid, you can predict which would be the expected bands. Partitioning is strictly controlled for low copy, but loose for high copy 8. Isolating, cloning, and sequencing dna molecular biology of the. Plasmids are small, circular, double stranded, nonchromosomal dna molecules. This will help you to prepare for what the exam will look like. What is a plasmid and describe unique characteristics of a plasmid. Lab cloning paper plasmid worksheet ms foglia ap biology. Plasmid mapping plasmids and restriction enzymes this lesson will demonstrate the principles of plasmid mapping by examining restriction digestion patterns of plasmids used in the laboratory section of the kit and determining the position of restriction enzyme recognition sites in the plasmids by use of logic. Since no plasmid was given to these bacteria, no growth is expected.
Scientists can typically distinguish cells that have been successfully transfected with a plasmid from those that have not by a. Although the newcomer likely knows that a plasmid is a small circular piece of dna found in bacterial cells, she may. Once you have selected those enzymes that cut the plasmid once, start checking these enzymes against the cell dna strand. A desktop resource 1st edition 2 p a g e plasmids 101. We also acknowledge previous national science foundation support under grant numbers 1246120, 1525057, and 14739. Construct a labeled map and explain 3 points maximum h h e e 40 20 30 10 h e 40 20 30 10 e h e ecori restriction point h haeiii restriction point. Restriction enzymes are specific to a section of dna, depending on the base pairs at that section, you will analzye sections of dna and determine which restriction enzyme should be used. Episome in bacteria, a plasmid that can be inserted into the chromosome. This technique allows molecular biologists to quickly evaluate the success of. Biology chapter 12 dna and rna worksheet answer key. Use a transformation solution containing cacl 2 ii. In the case of dna, this is feasible for relatively short molecules such as the genomes of small viruses.
This worksheet contains basic conceptual questions about the structure of dna. Plasmid must be unable to replicate without essential replication proteins provide in. In outbreaks, plasmid profiling is a rapid, convenient way to follow the spread. Emerging patterns of plasmidhost coevolution that stabilize. In bacterial cells, certain autonomously replicating circular genetic elements dna are found some. Manual poses a series of questions to focus and stimulate thinking about all. Structure of plasmid types of plasmid transfer of plasmid reproduction of plasmid 1. Direct student pairs to apply their understanding of genetics to modify plasmids. Locate the antibiotic resistant sites on the recombinant plasmid, along mth the replication site. Bacteria singlecelled microbes that were one of the first types of lifeforms to evolve on earth.
Using these data, construct a restriction map of plasmid. The action of these chemicals on the viral dna is shown in the diagram below. Ap biology 2007 scoring guidelines question 4 continued a using the circle provided, construct a labeled diagram of the restriction map of the plasmid. Below is a restriction map for the plasmid pgen101 total length 20 kb. Base your answers to questions 31 to 33 on the information below and on your howledge of biology. Use one or more complete sentences to answer each question. We biology lab cloning paper plasmid answer pdf on this page you can read or download lab cloning paper plasmid worksheet ms foglia ap biology in pdf format.
What is the first step in the cloning of a human gene. Good activity for an advanced biology or biotechnology class. This lecture note is specifically designed for medical laboratory technologists, and includes only those areas of molecular cell biology and applied genetics relevant to degreelevel understanding of modern laboratory technology. This quiz and worksheet combo can help you quickly determine your understanding of bacterial plasmids and the characteristics they have. Understand the uses of marker or reporter genes in molecular biology. The purpose of this technique is to introduce a foreign plasmid into bacteria, the bacteria. Describe how a plasmid can be genetically modified to include a piece of foreign dna that alters the phenotype of bacterial cells transformed with the modified plasmid. Jun 30, 2016 on this page you can read or download lab cloning paper plasmid worksheet ms foglia ap biology in pdf format. Since plasmid replicon type determines inc group, the terms inc and rep type were interchangeably used to describe plasmid types. Plasmids have played, and continue to play, indispensable roles in. Microsoft word ap bio lab06 molecular biology worksheet wl. To learn about bacterial plasmids, one of the basic tools of genetic engineering to purify plasmid dna from a bacterial culture. Using the word choices provided in the boxes, fill in the numbered boxes with the steps of bacteria. Following growth of the bacteria in the presence of the antibiotic, the plasmid dna can readily be isolated from the bacterial culture.
Pdf several pbluescriptderived plasmids of various sizes were constructed to study the effects of multicopy plasmid size on bacterial fitness. Plasmid analysis is a useful method for epidemiologic typing of a variety of organisms. Recover bacteria from the deceased animal spleen 4. Empirical and molecular formula worksheet an oxide of chromium is found to have the following % composition. Biology lab cloning paper plasmid answer pdf biology lab cloning paper plasmid answer pdf are you looking for ebook biology lab cloning paper plasmid answer pdf. Making recombinant dna molecules is at the heart of the genetic engineering controversy. Restriction enzymes teachers guide the biology corner. Dna strands restriction enzymes 36 34 32 30 28 26 24 22 20 18 16 14 12 10 8 6 4 2 0 rsa i sty i both. Recombinant paper plasmids cutandpaste biotechnology.
Students construct paper recombinant plasmids to simulate the methods genetic. Maybe dolly the sheep, or experiments carried out in molecular biology labs. Initially, inc typing relied on introduction of a plasmid into a strain carrying another plasmid and determining whether both plasmids were stably maintained in the progeny. Cbse worksheets for class 1 contains all the important questions on maths, english, hindi, moral science, social science, general knowledge, computers and environmental studies as per cbse syllabus.
Complete the remainder of the activity sheet you began on day 1 of the lab. But genomes of even the simplest cells are much too large to directly analyze in detail at the molecular level. Empirical formula worksheet ii wiggins elementary school. You will be tested on what domains plasmids can be found in and what the structure of a plasmid dna is.
Bacterial transformation with green fluorescent protein pglo genetic engineering is an umbrella term that encompasses many different techniques for moving dna between different organisms. Pdf the stable maintenance of lowcopynumber plasmids in bacteria is actively driven by partition mechanisms. What happens to the recombinant plasmid once inside a bacteria. Compare the output tag sequences with the input tags 5. Isolate plasmid dna and tag with a signature sequence 2. If you have no enzymes that will cut your plasmid only once, then reconstruct your plasmid. Keeping the flask with liquid agar in a water bath at 4550c can help prevent the agar from cooling too quickly. Plasmid replication requires host cell functions 9. Furthermore, within a single hostplasmid pair three distinct patterns of. Their presence influences, often dramatically, the biology of the organisms that they inhabit. On this page you can read or download ms foglia ap biology lab cloning paper plasmid answers in pdf format. Plasmid mapping has revolutionized molecular biology and paved the way for the biotechnology industry. The plasmid should be circular with a section of human dna spliced into the circle.
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